S4E2 - Choosing the right PCR method for your experiment

In this Mol Bio Minutes mini-episode of Speaking of Mol Bio, Dr. Andrea Hunger walks listeners through the practical differences between three core PCR approaches: endpoint PCR, quantitative PCR (qPCR), and digital PCR. Drawing on her experience in both academic research and industry, she explains how each technique provides different types of information and why choosing the right one depends on the biological question being asked. 

Endpoint PCR is the simplest method and is ideal for basic presence-or-absence questions such as confirming cloning success or genotyping samples. While fast and accessible, it does not provide quantitative information. For e...