S3E14 - Simplify your science ― why 1-step RT-PCR is a game changer

In this episode of Speaking of Mol Bio, Aistė Serapinaite, an experienced R&D scientist, shares her insights into the world of 1-Step RT-PCR—a method that simplifies RNA analysis by combining reverse transcription and PCR amplification in a single reaction. She explains how traditional RNA workflows once lengthy, multi-step processes were prone to errors and contamination, and how 1-Step RT-PCR has transformed this landscape with speed, efficiency, and fewer handling steps.

Listeners learn about the technical workings of 1-Step RT-PCR, including the importance of primer design, RNA quality, and essential controls to ensure reliable results. Aistė high...